Description
We are seeking a highly motivated Research Associate II to join our Protein Sciences team, supporting the discovery, engineering, and characterization of proteins. Reporting to the Principal Scientist, Protein Sciences, this individual will play a central role in molecular biology, protein expression, and functional screening work.
Key Responsibilities:
- Perform routine microbiology operations including media preparation, agar plate preparation, and aseptic technique
- Maintain rigorous good laboratory practices (GLP) and contribute to a strong culture of safety and operational excellence
- Generate and maintain microbial cell banks and inventory records
- Support routine laboratory maintenance, equipment upkeep, and troubleshooting
- Design and execute PCR for cloning, mutagenesis, and genotyping applications
- Perform DNA assembly using Golden Gate, Gibson, and other modern cloning techniques
- Run and analyze agarose gels; perform plasmid preparation (mini-prep, maxi-prep) and nucleic acid quantification
- Perform sequencing analysis using state-of-the-art NGS technologies and software
- Express engineered protein constructs across bacterial, yeast, and/or fungal expression systems
- Analyze protein expression, purity, and integrity via SDS-PAGE and/or Western blot
- Support protein purification workflows using standard chromatography and related techniques
- Support high-throughput screening workflows for functional characterization of protein variants
- Collaborate closely with the Protein Sciences team members and cross-functional partners
- Maintain clear, well-organized experimental records and communicate results effectively to the team
Professional Experience & Qualifications:
- Bachelor’s degree (with 2-5 years of relevant research experience) in Bioengineering, Biochemistry, Microbiology, Biotechnology, or related discipline
- Hands-on experience with core microbiology operations: media prep, agar plate prep, aseptic technique, and general GLP
- Strong hands-on experience with molecular cloning: design, PCR, gel electrophoresis and analysis, isolation, Golden Gate, Gibson assembly, and related techniques
- Experience with protein analysis techniques including SDS-PAGE and Western blot
- Strong communication, organizational and documentation skills
- Ability to independently execute experiments and troubleshoot routine issues