# Research Associate, Protein Production

**Company**: FL116
**Location**: Cambridge, MA
**Work arrangement**: onsite
**Experience**: mid
**Job type**: full-time
**Salary**: $65,000 - $93,500
**Category**: Research
**Industry**: Healthcare

**Apply**: https://job-boards.greenhouse.io/flagshippioneeringinc/jobs/8868157002?utm_source=yubhub.co&utm_medium=jobs_feed&utm_campaign=apply
**Canonical**: https://yubhub.co/jobs/job_680dcd71-f4d

## Description

We are seeking a highly motivated and exceptionally organized Research Associate II to expand our protein production capabilities across two complementary scales: bulk expression and purification of individual high-priority targets, and high-throughput parallel production of large variant panels.

This hands-on, laboratory-facing role will execute and advance scalable protein expression, purification, normalization, and characterization workflows that feed the design–build–test cycles at the core of our platform.

Key Responsibilities:

- Bulk Expression & Purification: Execute multi-liter recombinant expression campaigns in bacteria, design, build, and run preparative purification methods on ÄKTA FPLC systems, deliver milligram-to-gram quantities of highly pure, well-characterized protein.

- High-Throughput Production & Analytics: Execute miniaturized, parallel protein expression and purification in plate-based formats, perform automated purification, sample normalization, and quality assessment to deliver fit-for-purpose protein reagents at scale.

Required Qualifications & Experience:

- Bachelor’s degree in biochemistry, molecular biology, biotechnology, bioengineering, synthetic biology or a related scientific discipline with 1-4 years of relevant academic and/or industry experience; or a master’s degree with 0-2 years of commercial experience.

- Hands-on experience with recombinant protein expression and production workflows in E. coli, including multi-liter culture growth and harvest.

- Direct, hands-on experience operating ÄKTA FPLC systems, including independently designing, executing, and troubleshooting purification methods in UNICORN.

Preferred Qualifications:

- Experience with magnetic-bead–based purification platforms such as the Thermo KingFisher.

- Familiarity with cell-free or yeast expression systems.

- Hands-on experience programming and operating liquid handlers and robotic workstations.

The salary range for this role is $65,000 - $93,500. FL116 currently offers healthcare coverage, annual incentive program, retirement benefits and a broad range of other benefits.

## Skills

### Required
- recombinant protein expression
- protein purification
- ÄKTA FPLC systems
- UNICORN
- molecular biology
- biochemistry

### Nice to have
- magnetic-bead–based purification platforms
- cell-free expression systems
- liquid handlers
- robotic workstations

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Source: [Apply at job-boards.greenhouse.io](https://job-boards.greenhouse.io/flagshippioneeringinc/jobs/8868157002?utm_source=yubhub.co&utm_medium=jobs_feed&utm_campaign=apply)