# (Senior) Scientist, Translational Biology **Company**: FL106 Inc. **Location**: Cambridge, MA **Experience**: senior **Job type**: full-time **Salary**: $126,000 - $165,000 **Category**: Research **Industry**: Healthcare **Apply**: https://job-boards.greenhouse.io/flagshippioneeringinc/jobs/8861950002?utm_source=yubhub.co&utm_medium=jobs_feed&utm_campaign=apply **Canonical**: https://yubhub.co/jobs/job_5304f18d-d99 ## Description We are seeking a highly motivated and experienced Scientist to join our research team at FL106 Inc., a privately held, early-stage biotechnology company focused on developing novel drug delivery modalities. The ideal candidate will have deep expertise in flow cytometry, primary cell isolation and sorting, microscopy and imaging, and gene-silencing techniques (siRNA), with in vivo/in vitro mouse experience. Key Responsibilities: - Design, execute, and analyze complex in vitro / in vivo experiments to support research programs - Lead and perform multi-parameter flow cytometry assays, including panel design, optimization, and data interpretation - Perform and optimize primary cell isolation from tissues, including enzymatic and mechanical tissue dissociation, magnetic-activated cell sorting (MACS), and fluorescence-activated cell sorting (FACS), with attention to preserving cell viability, yield, native gene-expression signatures, and potential for further culture of the cells - Perform and interpret microscopy-based assays, including high-content microscopy, immunofluorescence, confocal, and/or intravital imaging, to characterize cell and tissue phenotypes and validate target engagement - Support mouse studies as needed, including disease models, dosing, tissue collection, and phenotypic analysis - Develop and apply siRNA-based approaches for target validation and mechanistic studies - Analyze and interpret data, generate reports, and present findings to cross-functional teams - Collaborate with internal and external stakeholders to advance project goals - Mentor junior scientists and contribute to a collaborative team environment - Maintain accurate experimental records and ensure compliance with institutional and regulatory guidelines Qualifications: - Ph.D. in Molecular Biology, Cell Biology, or a related field with 3–6+ years of relevant experience (industry preferred), or M.S. with significant experience - Strong expertise in flow cytometry, including cell sorting (FACS), panel design, troubleshooting, and data analysis (e.g., FlowJo or similar tools) - Hands-on experience with primary cell isolation techniques from mouse tissue, including tissue dissociation (enzymatic and mechanical), magnetic bead-based separation (MACS), and isolation of rare or fragile cell populations - Hands-on experience with microscopy techniques (e.g., immunofluorescence, confocal microscopy) and associated image analysis software (e.g., ImageJ/Fiji, Imaris, or similar) - Demonstrated experience with siRNA design, delivery, and functional validation - Strong data analysis and problem-solving skills - Excellent communication and presentation skills - Ability to work independently and collaboratively in a fast-paced environment Preferred Qualifications: - Experience with mouse models, including in vivo study design, dosing, and tissue collection - Experience in translational or preclinical research environments - Experience with cold-active or optimized dissociation protocols for isolating sensitive/rare cell types (e.g., podocytes, neurons, microglia) while minimizing dissociation-induced expression artifacts - Experience with advanced imaging modalities (e.g., intravital microscopy, super-resolution, high-content imaging) - Familiarity with additional molecular techniques (qPCR, Western blot, ELISA, RNA-seq, gene silencing, protein over-expression) - Experience working in a biotech or pharmaceutical setting The salary range for this role is $126,000 - $165,000. FL106 currently offers healthcare coverage, annual incentive program, retirement benefits and a broad range of other benefits. ## Skills ### Required - flow cytometry - primary cell isolation - microscopy - siRNA - gene-silencing techniques - in vivo/in vitro mouse experience ### Nice to have - mouse models - translational research - preclinical research - advanced imaging modalities - molecular techniques --- Source: [Apply at job-boards.greenhouse.io](https://job-boards.greenhouse.io/flagshippioneeringinc/jobs/8861950002?utm_source=yubhub.co&utm_medium=jobs_feed&utm_campaign=apply)