# Research Associate II, Protein Sciences

**Company**: Flagship Pioneering
**Location**: Cambridge, MA
**Work arrangement**: onsite
**Experience**: mid
**Job type**: full-time
**Salary**: $74,000 - $93,500
**Category**: Research
**Industry**: Healthcare

**Apply**: https://job-boards.greenhouse.io/flagshippioneeringinc/jobs/8882558002?utm_source=yubhub.co&utm_medium=jobs_feed&utm_campaign=apply
**Canonical**: https://yubhub.co/jobs/job_17dbda42-a8f

## Description

We are seeking a highly motivated Research Associate II to join our Protein Sciences team, supporting the discovery, engineering, and characterization of proteins. Reporting to the Principal Scientist, Protein Sciences, this individual will play a central role in molecular biology, protein expression, and functional screening work.

Key Responsibilities:

- Perform routine microbiology operations including media preparation, agar plate preparation, and aseptic technique

- Maintain rigorous good laboratory practices (GLP) and contribute to a strong culture of safety and operational excellence

- Generate and maintain microbial cell banks and inventory records

- Support routine laboratory maintenance, equipment upkeep, and troubleshooting

- Design and execute PCR for cloning, mutagenesis, and genotyping applications

- Perform DNA assembly using Golden Gate, Gibson, and other modern cloning techniques

- Run and analyze agarose gels; perform plasmid preparation (mini-prep, maxi-prep) and nucleic acid quantification

- Perform sequencing analysis using state-of-the-art NGS technologies and software

- Express engineered protein constructs across bacterial, yeast, and/or fungal expression systems

- Analyze protein expression, purity, and integrity via SDS-PAGE and/or Western blot

- Support protein purification workflows using standard chromatography and related techniques

- Support high-throughput screening workflows for functional characterization of protein variants

- Collaborate closely with the Protein Sciences team members and cross-functional partners

- Maintain clear, well-organized experimental records and communicate results effectively to the team

Professional Experience & Qualifications:

- Bachelor’s degree (with 2-5 years of relevant research experience) in Bioengineering, Biochemistry, Microbiology, Biotechnology, or related discipline

- Hands-on experience with core microbiology operations: media prep, agar plate prep, aseptic technique, and general GLP

- Strong hands-on experience with molecular cloning: design, PCR, gel electrophoresis and analysis, isolation, Golden Gate, Gibson assembly, and related techniques

- Experience with protein analysis techniques including SDS-PAGE and Western blot

- Strong communication, organizational and documentation skills

- Ability to independently execute experiments and troubleshoot routine issues

## Skills

### Required
- microbiology
- molecular cloning
- protein analysis
- DNA assembly
- PCR
- gel electrophoresis
- plasmid preparation
- sequencing analysis
- protein expression
- purification
- chromatography
- high-throughput screening

### Nice to have
- bioinformatics
- ELN tools
- Benchling
- Geneious
- protein engineering
- functional screening campaigns

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Source: [Apply at job-boards.greenhouse.io](https://job-boards.greenhouse.io/flagshippioneeringinc/jobs/8882558002?utm_source=yubhub.co&utm_medium=jobs_feed&utm_campaign=apply)